|本期目录/Table of Contents|

[1]李必强,林威,姚恩辉,等.小檗碱逆转高糖对血管内皮细胞迁移的抑制作用[J].福建医科大学学报,2013,47(06):350-353.
 LI Biqiang,LIN Wei,YAO Enhui,et al.Berberine Reverses the Effect of High Dglucose on Endothelial Cells Migration[J].Journal of Fujian Medical University,2013,47(06):350-353.
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第47卷
期数:
2013年06期
页码:
350-353
栏目:
论著
出版日期:
2013-12-31

文章信息/Info

Title:
Berberine Reverses the Effect of High Dglucose on Endothelial Cells Migration
文章编号:
1672-4194(2013)06-0350-04
作者:
李必强1 林威1 姚恩辉2 林楠1 黄培基3
福建医科大学 附属协和医院,福州3500011.干部病房;
2.心内科;
3.内分泌科
Author(s):
LI Biqiang1 LIN Wei1 YAO Enhui2 LIN Nan1 HUANG Peiji3
1. Cadres Ward;
2. Department of Cardiology;
3. Department of Endocrinology, Fujian Medical University Union Hospital, Fuzhou350001, China
关键词:
糖尿病 内皮血管 内皮细胞 小檗碱 核因子κB
Keywords:
Diabetes Mellitus endothelium vascular endothelial cells Berberine NFkappa B
分类号:
R284.1
DOI:
-
文献标志码:
A
摘要:
目的观察小檗碱对高糖条件下血管内皮细胞迁移的影响,并研究可能的作用机制。方法以体外培养的EA.hy926人脐静脉血管内皮细胞为模型,采用划痕试验的方法研究高糖及不同浓度条件下对内皮细胞迁移的影响;采用电泳迁移率变动分析法检测核因子κB(NFκB)的DNA结合活性。结果33 mmol/L浓度的葡萄糖可抑制内皮细胞的迁移;小檗碱可逆转高糖对内皮细胞迁移的抑制作用。作用机制的研究表明,高糖可诱导NFκB DNA结合活性上调,而采用小檗碱处理后可降低NFκB活性。结论小檗碱可逆转高糖对内皮细胞迁移的抑制作用,该作用可能涉及抑制NFκB的DNA结合活性上调。
Abstract:
ObjectiveTo investigate the effect of berberine on the migration of endothelial cells treated by high glucose, and explore the potential mechanisms.MethodsEA.hy926 cell line was used in the present study.Cells were treated with 33 mmol/L Dglucose alone or combined with different concentrations of berberine; the migration rate of endothelial cells was measured by wound scratch assay.DNA binding activity of NFκB was detected by using electrophoretic mobility shift assay (EMSA).Results33 mmol/L Dglucose inhibited the migration of endothelial cells, whereas berberine reversed the effect of high glucose on endothelial cells.Further mechanism studies showed that high glucose upregulated the activity of NFκB.Berberine inhibited the upregulation of NFκB activity induced by high glucose.ConclusionBerberine reverses the inhibition of Dglucose on endothelial cells, which might be associated with the suppression of upregulation of NFκB activity.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2013-08-26
作者单位: 福建医科大学 附属协和医院,福州3500011.干部病房;2.心内科;3.内分泌科
作者简介: 李必强(1967-),男,副主任医师. Email:lbq387@126.com
更新日期/Last Update: 2013-12-30