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[1]林俊,朱益华.Brn-3a标记大鼠视网膜神经节细胞的实验研究[J].福建医科大学学报,2014,48(02):75-78.
 LIN Jun,ZHU Yihua.An Experimental Study on Brn-3a as a Marker of Retinal Ganglion Cells[J].Journal of Fujian Medical University,2014,48(02):75-78.
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Brn-3a标记大鼠视网膜神经节细胞的实验研究(PDF)
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第48卷
期数:
2014年02期
页码:
75-78
栏目:
论著
出版日期:
2014-12-27

文章信息/Info

Title:
An Experimental Study on Brn-3a as a Marker of Retinal Ganglion Cells
文章编号:
1672-4194(2014)02-0075-04
作者:
林俊 朱益华
福建医科大学 附属第一医院眼科,福州350005
Author(s):
LIN Jun ZHU Yihua
Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China
关键词:
青光眼 神经系统 神经元 视网膜神经节细胞 转录因子Brn-3a 抗原Thy-1 染色与标记 免疫组织化学 疾病模型动物
Keywords:
glaucoma nervous system neurons retinal ganglion cells transcription factor Brn-3a antigens Thy-1 staining and labeling immunohistochemistry disease models animal
分类号:
R332
DOI:
-
文献标志码:
A
摘要:
目的对比Thy-1.1标记大鼠视网膜神经节细胞(RGCs)的方法,评估Brn-3a是否可作为一种可靠的内源性标记物,标记并计数大鼠RGCs。方法20只SD大鼠,随机选取大鼠一眼为Thy-1.1组,另一眼作为Brn-3a组,常规H-E染色镜下观察RGCs的形态,运用免疫组织化学法检测Brn-3a与Thy-1.1的表达情况,每只眼球取1张切片,在每张待测视网膜切片上,以视网膜后极部中点为起点,至近角膜缘视网膜止点处,将视网膜分为3等份,即中央区、中间区及周边区,每个区域视网膜随机取5个高倍视野,分别计数Thy-1.1、Brn-3a阳性细胞均数。结果光镜下正常大鼠的视网膜从内向外可见3个细胞核层,依次为神经节细胞层(GCL)、内核层(INL)、外核层(ONL)。GCL的细胞呈单层排列,较为整齐,胞核清楚,数目相对较少,INL和ONL呈多层排列,且细胞排列紧密,数目相对较多。2种标记物染色都仅仅存在于视网膜的GCL,但在大鼠RGCs中分布呈明显差异:Brn-3a主要在胞核中着色,Thy-1.1主要在胞质中着色;运用此2种标记物标记计数RGCs,各个相同区域内Thy-1.1和Brn-3a阳性细胞计数比较,差别均无统计学意义(P>0.05)。Thy-1.1组和Brn-3a组中央区、中间区和周边区两两比较,差别均有统计学意义(P<0.01)。结论在大鼠视网膜中,Brn-3a可以作为一种比较可靠的内源性标记物,标记并计数大鼠RGCs。
Abstract:
ObjectiveTo identify Brn-3a is a reliable endogenous marker of RGCs by contrast with Thy-1.1 expression of the characteristics and differences count in rats.Methods20 health adult SD rats were divided into two groups, one eye was selected randomly for group of Thy-1.1, at the other as group of Brn-3a in the same rat.Morphological structure was investigated by haematotoxylin-eosine staining of retinal paraflin section respectively.Expression of Brn-3a and Thy-1.1 was measured by immunohistochemical method.RGCs were counted by immunolabelled cells.ResultsThere are ganglion cell layer(GCL), inner nuclear layer(INL) and outer nuclear layer(ONL) in retina of rat.GCL was monolayer, alignment rarefaction.Brn-3a+ cells were only present in the GCL and showed a spatial distribution comparable to that of Thy-1.1+ cells: the Brn-3a signal was located in the nuclei, and the Thy-1.1 signal was major in the cytoplasm.There was no significant difference between the numbers of RCGs by labeling of these two markers in the same area.There was a significant difference among in the numbers of RCGs by labeling of these two markers in the different area.ConclusionBrn-3a can be used as a reliable marker to identify and quantify RGCs.

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备注/Memo

备注/Memo:
收稿日期: 2014-02-21
基金项目: 国家自然科学基金(81270999);福建省医学创新课题(2012-CX-24)
作者单位: 福建医科大学 附属第一医院眼科,福州350005
作者简介: 林俊(1986-),男,住院医师,医学硕士
通讯作者: 朱益华. Email:zhuyihua889@126.com
更新日期/Last Update: 2014-04-30