|本期目录/Table of Contents|

[1]耿玉聪,高学敏,李世峰,等.乙酰化微管蛋白α在肺、肝和肾纤维化中的表达及其病理意义[J].福建医科大学学报,2016,50(04):232-236.
 GENG Yucong,GAO Xuemin,LI Shifeng,et al.The Expression and Pathological Significances ofAc-α-Tub in Lung, Liver and Renal Fibrosis[J].Journal of Fujian Medical University,2016,50(04):232-236.
点击复制

乙酰化微管蛋白α在肺、肝和肾纤维化中的表达及其病理意义(PDF)
分享到:

《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第50卷
期数:
2016年04期
页码:
232-236
栏目:
论著
出版日期:
2016-08-30

文章信息/Info

Title:
The Expression and Pathological Significances ofAc-α-Tub in Lung, Liver and Renal Fibrosis
文章编号:
1672-4194(2016)04-0232-05
作者:
耿玉聪1 高学敏1 李世峰1 徐丁洁2 徐 洪1 杨 方1
华北理工大学,唐山 063000 1. 医学实验研究中心;
2. 医学中医学院
Author(s):
GENG Yucong1 GAO Xuemin1 LI Shifeng1 XU Dingjie2 XU Hong1 YANG Fang1
1. Medical Research Center;
2. College of Chinese Traditional Medicine,North China University of Science and Technology, Tangshan 063000, China
关键词:
微管蛋白 乙酰化作用 纤维化 免疫组织化学 染色与标记
Keywords:
tubulin acetylation fibrosis immunohistochemistry staining and labeling
分类号:
R135.2; R693.2
DOI:
-
文献标志码:
A
摘要:
目的 研究乙酰化微管蛋白α(Ac-α-Tub)在动式染尘矽肺大鼠肺纤维化模型、单侧输尿管结扎(UUO)肾纤维化模型和硫代乙酰胺(TAA)致肝纤维化模型中的表达及其病理意义。 方法 采用动式染尘构建矽肺大鼠模型,采用UUO构建肾纤维化模型,采用TAA构建肝纤维化模型。采用免疫组织化学染色观察Ac-α-Tub及波形蛋白(vimentin)在组织中的差异表达,采用HistoQurst图像分析软件对免疫组织化学结果进行分析。 结果 随着染尘时间的延长,vimentin的阳性表达率明显上调,而Ac-α-Tub的阳性表达率明显下调; 在肝、肾纤维化中,随着造模时间的延长,vimentin和Ac-α-Tub的阳性表达率均明显上调。 结论 Ac-α-Tub在肺、肝和肾纤维化中阳性细胞的定位及临床病理意义均具有差异,其缺失表达可能提示肺纤维化进展,而在肝、肾纤维化过程中则可能起到相反作用。
Abstract:
Objective To observe the expression and pathological significance of acetylated α-tubulin(Ac-α-Tub)in rats with silicosis, unilateral ureteral obstruction(UUO)and thioacetamide(TAA). Methods Silicosis model was established with inhalation of d SiO2 in a dynamic manner. Renal fibrosis model was established by UUO. Hepatic fibrosis model was established by TAA intraperitoneal injection. The expression of Ac-α-Tub and vimentin were measured by immunohistochemistry staining and analyzed by HistoQurst software. Results With the time of dust exposure extended, the positive rate of vimentin was increased accompanying a down-regulation of Ac-α-Tub. In the renal and hepatic fibrosis, the positive rate of Ac-α-Tub and vimentin was all increased with the extension of the time of models creation. Conclusion There is a differential expression of Ac-α-Tub and pathological significance in lung, renal and hepatic fibrosis. The down-expression of Ac-α-Tub in silicosis, and up-expression of it in UUO and TAA in rats.

参考文献/References:

[1] 徐 洪, 孙 月, 徐丁洁, 等. α-平滑肌肌动蛋白、波形蛋白在大鼠矽肺模型中表达的病理形态学特点及其意义[J]. 解剖学杂志, 2014,37(3):300-303.
[2] 李世峰, 高学敏, 徐丁洁, 等. A-SDKP调节乙酰化微管蛋白α抑制矽肺肌成纤维细胞转化的研究[J]. 中华劳动卫生职业病杂志, 2015,33(11):816-821.
[3] Yuan Y, Zhang F, Wu J, et al. Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model[J]. Sci Rep, 2015,5:9314.
[4] Schlederer M, Mueller K M, Haybaeck J, et al. Reliable quantification of protein expression and cellular localization in histological sections[J]. PLoS One, 2014,9(7):e100822.
[5] Perdiz D, Mackeh R, Poüs C, et al. The ins and outs of tubulin acetylation: more than just a post-translational modification[J]? Cell Signal, 2011,23(5):763-771.
[6] Bulinski J C, Richards J E, Piperno G. Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase microtubules in cultured cells[J]. J Cell Biol, 1988,106(4):1213-1220.
[7] Kim J I, Kim J, Jang H S, et al. Reduction of oxidative stress during recovery accelerates normalization of primary cilia length that is altered after ischemic injury in murine kidneys[J]. Am J Physiol Renal Physiol, 2013,304(10):1283-1294.
[8] Maggiorani D, Dissard R, Belloy M, et al. Shear stress-induced alteration of epithelial organization in human renal tubular cells[J]. PLoS One, 2015,10(7):e0131416.
[9] Wang S, Livingston M J, Su Y, et al. Reciprocal regulation of cilia and autophagy via the MTOR and proteasome pathways[J]. Autophagy, 2015,11(4):607-616.
[10] Kannarkat G T, Tuma D J, Tuma P L. Microtubules are more stable and more highly acetylated in ethanol-treated hepatic cells[J]. J Hepatol, 2006,44(5):963-970.
[11] Shan B, Yao T P, Nguyen H T, et al. Requirement of HDAC6 for transforming growth factor-beta1-induced epithelial-mesenchymal transition [J]. J Biol Chem, 2008,283(30):21065-21073.
[12] Wang Z, Chen C, Finger S N, et al. Suberoylanilide hydroxamic acid: a potential epigenetic therapeutic agent for lung fibrosis [J]? Eur Respir J, 2009,34(1):145-155.
[13] Hashimoto-Komatsu A, Hirase T, Asaka M, et al. Angiotensin Ⅱ induces microtubule reorganization mediated by a deacetylase SIRT2 in endothelial cells [J]. Hypertens Res, 2011, 34(8): 949-956.
[14] Thompson K L, Assoian R, Rosner M R.Transforming growth factor-beta increases transcription of the genes encoding the epidermal growth factor receptor and fibronectin in normal rat kidney fibroblasts [J]. J Biol Chem, 1988,263(36):19519-19524.

相似文献/References:

[1]张惠,徐丁洁,毛娜,等.乙酰化微管蛋白α缺失表达在矽肺上皮-间质转化中的意义[J].福建医科大学学报,2018,52(04):229.
 ZHANG Hui,XU Dingjie,MAO Na,et al.The Mechanism of Epithelial-Mesenchymal Transition in Silicosis Regulated by Loss Expression of Acetylated Tubulin α[J].Journal of Fujian Medical University,2018,52(04):229.

备注/Memo

备注/Memo:
收稿日期: 2016-04-16基金项目: 国家自然科学基金(81472953); 河北省自然科学基金(H201409115); 河北省研究生创新项目(2015S04)作者单位: 华北理工大学,唐山 063000 1. 医学实验研究中心; 2. 医学中医学院作者简介: 耿玉聪(1989-),女,华北理工大学2015级硕士研究生通讯作者: 杨 方. Email: fangyang1955@163.com
更新日期/Last Update: 2016-08-30