|本期目录/Table of Contents|

[1]许静,李姗,蔡俊玮,等.miR|146a及其靶mRNA与初发Graves病的相关性研究[J].福建医科大学学报,2019,53(05):291-296.
 XU Jing,LI Shan,CAI Junwei,et al.Down|Regulation of p21 Expression[J].Journal of Fujian Medical University,2019,53(05):291-296.
点击复制

miR|146a及其靶mRNA与初发Graves病的相关性研究(PDF)
分享到:

《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第53卷
期数:
2019年05期
页码:
291-296
栏目:
论著
出版日期:
2019-10-31

文章信息/Info

Title:
Down|Regulation of p21 Expression
文章编号:
1672-4194(2019)05-0291-06
作者:
许静1 李姗2 蔡俊玮1 黄成虎1 龚丽1 李敏1 李雪锋1
湖北医药学院 附属医院,十堰市太和医院,十堰4420001. 内分泌科;2. 感染与免疫研究所
Author(s):
XU Jing1 LI Shan2 CAI Junwei1 HUANG Chenghu1 GONG Li1 LI Min1 LI Xuefeng1
1. Department of Endocrinology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China;2. Institute of Infection and Immunity, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
关键词:
格雷夫斯病 微RNA RNA信使 白细胞介素8 趋化因子CCL8
Keywords:
graves disease microRNA RNA messenger interleukin|8 chemokine CCL8
分类号:
R342.2; R392.11; R581.1
DOI:
-
文献标志码:
-
摘要:
目的探讨微小RNA|146a(miR|146a)及其靶mRNA在初发Graves病(GD)患者外周血单个核细胞(PBMC)中的表达情况及临床意义。方法采用实时定量聚合酶链式反应(RT|PCR)方法,检测30例初发GD和26例健康对照者PBMC中miR|146a及其靶mRNA的相对表达水平,并与各临床因子进行相关性分析。结果(1)相对于对照组,初发GD患者PBMC中miR|146a表达量降低(P<0.01),白细胞介素|8(IL|8)和CC趋化因子配体8(CCL8)的mRNA的表达量升高(P<0.01)。(2)miR|146a的表达水平与IL|8的mRNA、CCL8的mRNA以及血清总三碘甲状腺原氨酸(TT3)、总四碘甲状腺原氨酸(TT4)、游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)、促甲状腺激素受体抗体(TRAb)水平均呈负相关,与血清促甲状腺激素(TSH)水平呈正相关,而IL|8和CCL8的mRNA表达水平与上述临床指标的关系则相反。结论miR|146a可能通过抑制IL|8和CCL8的mRNA的表达而在GD的发生中发挥作用。
Abstract:
ObjectiveTo investigate the expression and clinical significance of microRNA|146a(miR|146a) and its immune|related target mRNA in peripheral blood mononuclear cells(PBMCs)in patients with Graves′ disease (GD).MethodsPeripheral blood from 30 primary GD patients and 26 healthy donors were collected.Quantitative Real|time polymerase chain reaction(RT|PCR)was performed to determine the relative expression levels of miR|146a and its target mRNA in PBMCs,and their correlation with clinical factors was also analyzed.Results(1) Compared with the healthy controls, the expression levels of miR|146a in PBMCs in primary GD patients were significantly decreased (P<0.01), while the expression levels of IL|8(interleukin|8) and CCL8 [chemokine ( C|C motif) ligand 8]were significantly increased (P<0.01).(2) The expression level of miR|146a was negatively correlated with IL|8 and CCL8, as well as the serum TT3, TT4, FT3, FT4, and TRAb (P<0.05), but positively correlated with TSH (P<0.01),while IL|8 and CCL8 were opposite to it(P<0.05).ConclusionmiR|146a may play a role in the occurrence of GD by inhibiting the expression of mRNAs of IL|8 and CCL8.

参考文献/References:

[1]Burch H B, Cooper D S. Management of Graves disease[J]. JAMA, 2015,314(23):2544.[2]Selvamani S P, Mishra R, Singh S K. Chikungunya virus exploits miR|146a to regulate NF|κB pathway in human synovial fibroblasts[J]. PLoS One, 2014,9(8): e103624.[3]Pfeiffer D, Rossmanith E, Lang I, et al. miR|146a, miR|146b, and miR|155 increase expression of IL|6 and IL|8 and support HSP10 in an in vitro sepsis model[J]. PLoS One, 2017,12(6):e179850.[4]Rom S, Rom I, Passiatore G, et al. CCL8/MCP|2 is a target for miR|146a in HIV|1|infected human microglial cells[J]. The FASEB J, 2010,24(7):2292|2300.[5]Huang Q, Chen L, Luo M, et al. HIV|1|induced miR|146a attenuates monocyte migration by targeting CCL5 in human primary macrophages[J]. AIDS Res Hum Retroviruses, 2018,34(7):580|589.[6]Sandhu R, Rein J, D Arcy M, et al. Overexpression of miR|146a in basal|like breast cancer cells confers enhanced tumorigenic potential in association with altered p53 status[J]. J Carcinog, 2014,35(11):2567|2575.[7]郑慧娟, 魏璠, 魏军平. Graves病发病机制新进展[J]. 中国免疫学杂志, 2017,33(4):621|624.[8]Li J, Huang J, Dai L, et al. miR|146a, an IL|1b responsive miRNA, induces vascular endothelial growth factor and chondrocyte apoptosis by targeting Smad4[J]. Arthritis Res Ther, 2012,14(2):R75.[9]Ammari M, Presumey J, Ponsolles C, et al. Delivery of miR|146a to Ly6C(high) monocytes inhibits pathogenic bone erosion in inflammatory arthritis[J]. Theranostics, 2018,8(21):5972|5985.[10]Shumnalieva R, Kachakova D, Shoumnalieva|Ivanova V, et al.Whole peripheral blood miR|146a and miR|155 expression levels in systemic lupus erythematosus patients[J]. Acta Reumatol Port, 2018,43(3):217|225.[11]Zilahi E, Tarr T, Papp G, et al. Increased microRNA|146a/b,TRAF6 gene and decreased IRAK1 gene expressions in the peripheral mononuclear cells of patients with Sjgren′s syndrome[J]. Immunology Lett, 2012,141(2):165|168.[12]Assmann T S, Duarte G, Brondani L A, et al. Polymorphisms in genes encoding miR|155 and miR|146a are associated with protection to type 1 diabetes mellitus[J]. Acta Diabetol, 2017,54(5):433|441.[13]Otsu H, Watanabe M, Inoue N, et al. Intraindividual variation of microRNA expression levels in plasma and peripheral blood mononuclear cells and the associations of these levels with the pathogenesis of autoimmune thyroid diseases[J]. CCLM, 2017,55(5):626|635.[14]Wang N, Chen F E, Long Z W. Mechanism of microRNA|146a/Notch2 signaling regulating IL|6 in Graves ophthalmopathy[J]. Cell Physiol Biochem, 2017,41(4):1285|1297.[15]Bernecker C, Lenz L, Ostapczuk M S, et al. MicroRNAsmiR|146a1, miR|155_2, and miR|200a1 are regulated in autoimmune thyroid diseases[J]. Thyroid, 2012,22(12):1294|1295.[16]Jang S Y, Chae M K, Lee J H, et al. Role of miR|146a in the regulation of inflammation in an in vitro model of Graves′ orbitopathy[J]. Invest Ophthalmol Vis Sci, 2016,57(10):4027|4034.[17]Zheng L, Zhuang C, Wang X, et al. Serum miR|146a, miR|155, and miR|210 as potential markers of Graves′ disease[J]. J Clin Lab Anal, 2018,32(2): e22266.[18]杨文娟, 班胜刚, 何剑峰. 甲状腺相关眼病患者外周血单个核细胞中微小RNA|146a的异常表达及意义[J]. 眼科新进展, 2012,32(5):414|417.[19]李瑶, 吕德官, 陈临溪. IL|8及其受体药物与疾病的研究进展[J]. 中国药理学通报, 2014,30(3):310|314.[20]Wei H, Guan M, Qin Y, et al. Circulating levels of miR|146a and IL|17 are significantly correlated with the clinical activity of Graves′ ophthalmopathy[J]. Endocr J, 2014,61(11):1087|1092.[21]刘颖, 赵令君, 潘春枝, 等. Graves病患者131I治疗前后血清IL|8水平的变化及相关研究[J]. 标记免疫分析与临床, 2009,16(6):346|350.[22]Wang N, Chen F, Long Z. Mechanism of microRNA|146a/Notch2 signaling regulating IL|6 in Graves′ ophthalmopathy[J].Cell Physiol Biochem, 2017,41(4):1285|1297.[23]Jang S Y, Chae M K, Lee J H, et al. Role of miR|146a in the regulation of inflammation in an in vitro model of Graves′ orbitopathy[J]. Inves Opthalmol Vis Sci, 2016,57(10):4027.[24]Chen H, Mester T, Raychaudhuri N, et al. Teprotumumab, an IGF|1R blocking monoclonal antibody inhibits TSH and IGF|1 action in fibrocytes[J]. J Clin Endocrinol Metab, 2014,99(9): E1635|E1640.[25]周倩, 张国龙, 王秀丽. CCL8在疾病免疫调节中的作用[J].国际免疫学杂志, 2016,39(3):250|253.

相似文献/References:

[1]许 诺,崔 乙,郑 宓,等.甲状腺相关眼病眼眶炎症与眼表功能关系探讨[J].福建医科大学学报,2017,51(02):113.
 XU Nuo,CUI Yi,ZHENG Mi,et al.Correlation between Orbit Inflammation and Ocular SurfaceCharacteristic in Thyroid Associated Ophthalmopathy[J].Journal of Fujian Medical University,2017,51(05):113.
[2]陈恩,蔡炜,吴佳易,等.miR|208a通过下调p21的表达促进心肌肥厚[J].福建医科大学学报,2019,53(05):285.
 CHEN En,CAI Wei,WU Jiayi,et al.microRNA|208a Promotes Cardiac Hypertrophy through[J].Journal of Fujian Medical University,2019,53(05):285.
[3]唐平易,张琳,唐东兴,等.正常白蛋白尿期糖尿病患者血清miR|16|5p与FGF2的相关性及在肾损伤随访中的意义[J].福建医科大学学报,2019,53(05):328.
 TANG Pingyi,ZHANG Lin,TANG Dongxing,et al.The Correlation between Serum microRNA|16|5p and FGF2 in Patients withNormal Albuminuria and its Significance in Follow|up of Renal Injury[J].Journal of Fujian Medical University,2019,53(05):328.

备注/Memo

备注/Memo:
收稿日期:2018|12|14基金项目: 国家自然科学基金(81372998);十堰市科技局科学技术研究与开发项目(16Y15)作者简介: 许静,女,湖北医药学院2016级硕士研究生通讯作者: 李雪锋. Email: ymclixf@163.com
更新日期/Last Update: 2019-10-25