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[1]方 玲,黄惠萍,杨珍珍,等.便携式血脂仪与全自动生化仪检测卒中患者 低密度脂蛋白胆固醇的比对分析[J].福建医科大学学报,2020,54(01):23-28.
 FANG Ling,HUANG Huiping,YANG Zhenzhen,et al.Comparison of Low Density Lipoprotein Cholesterol in Ischemic Stroke Detected by CardioChek PA Analyzer and ADVIA2400 Automatic Analyzer[J].Journal of Fujian Medical University,2020,54(01):23-28.
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第54卷
期数:
2020年01期
页码:
23-28
栏目:
临床研究
出版日期:
2020-02-28

文章信息/Info

Title:
Comparison of Low Density Lipoprotein Cholesterol in Ischemic Stroke Detected by CardioChek PA Analyzer and ADVIA2400 Automatic Analyzer
文章编号:
1672-4194(2020)01-0023-06
作者:
方 玲12 黄惠萍1 杨珍珍1 郭祈福1 吴 华1
1.福建医科大学 附属第一医院神经内科,福州 350005; 2.福建省神经分子重点实验室,福州 350005
Author(s):
FANG Ling12 HUANG Huiping1 YANG Zhenzhen1 GUO Qifu1 WU Hua1
1. Department of Neurology,The First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China; 2. Fujian Key Laboratory of Molecular Neurology,Fuzhou 350005,China
关键词:
脑缺血 卒中 脂蛋白类 LDL 床旁诊断化验信息系统
Keywords:
brain ischemia stroke lipoproteins LDL point-of-care systems
分类号:
R446; R743.3; R743.31
DOI:
-
文献标志码:
A
摘要:
目的 比较CardioChek PA便携式血脂仪与全自动生化仪检测卒中患者低密度脂蛋白胆固醇(LDL-C)的差异,评估两者的相关性及偏差。 方法 选择82例非心源性急性缺血性脑卒中住院患者,清晨空腹卧位指尖采血,以CardioChek PA血脂仪检测末梢全血的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及LDL-C血脂4项,并在5 min内抽取受试者肘静脉血,分别采用西门子ADVIA2400全自动生化仪检测血清血脂4项,CardioChek PA血脂仪检测静脉全血的血脂4项。将CardioChek PA血脂仪检测末梢全血或静脉全血的血脂数值与ADVIA2400全自动生化仪检测结果进行统计分析,计算两种检测系统血脂4项数值的相关系数、直线回归方程、血脂异常检出率及偏倚,并用标准化西格玛性能验证图评价CardioChek PA血脂仪检测LDL-C的性能。 结果 ADVIA2400自动生化分析仪直接法检测LDL-C为(2.92±1.02)mmol/L,CardioChek PA血脂仪提供末梢全血LDL-C为(2.29±0.99)mmol/L,静脉全血LDL-C为(2.20±1.00)mmol/L,CardioChek PA血脂仪的LDL-C数值均低于全自动生化仪直接检测值,差别有统计学意义(P<0.05)。应用CardioChek PA血脂仪检测末梢全血或静脉全血LDL-C数值差别无统计学意义(P>0.05)。直线回归显示,LDL-C的ADVIA2400全自动生化分析仪组与CardioChek PA血脂仪末梢全血组相关系数为0.861(P<0.001),与静脉全血组相关系数为0.831(P<0.001)。应用CardioChek PA血脂仪的高TC、高TG及低HDL-C检出率与全自动生化仪比较无差异,但高LDL-C的检出率低于全自动生化仪(P<0.05)。标准化西格玛性能验证图评价CardioChek PA血脂仪检测LDL-C的性能为不可接受(σ<2)。 结论 CardioChek PA血脂仪的采血方式不影响LDL-C检测结果,应用该便携式血脂仪检测缺血性卒中患者的LDL-C值低于全自动生化仪,故缺血性卒中患者的临床决策仍需参照全自动生化分析仪检测的LDL-C数值。
Abstract:
Objective Compare the difference of low density lipoprotein cholesterol(LDL-C)in stroke patients between a point-of-care device CardioChek PA analyzer and ADVIA2400 automatic analyzer, and to evaluate the correlation and deviation between them. Methods Blood samples were collected in the morning from 82 patients of noncardioembolic ischemic stroke in the department of neurology during April to June 2015. The total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C), and LDL-C were detected by CardioChek PA analyzer from the periphery or elbow venous blood samples immediately, and then detected by Siemens ADVIA2400 automatic analyzer from venous serum. The correlation coefficient and linear regression equations as well as rates of hyperlipidemia and bias were calculated,and sigma performance verification were used to evaluate the testing projects. Results The outcome showed that the values of LDL-C were(2.29±0.99)mmol/L(peripheral blood)and(2.20±1.00)mmol/L(venous blood)as detected by CardioChek PA analyzer, and these values were lower than that of ADVIA2400 automatic analyzer which is(2.92±1.02)mmol/L(P<0.05). The values of LDL-C did not have significant difference between peripheral blood and venous samples by CardioChek PA analyzer. Linear regression analysis showed the correlation coefficients of LDL-C were 0.861 and 0.831 between peripheral blood or venous samples by CardioChek PA analyzer and ADVIA2400 automatic analyzer(P<0.001). The rates of high TC, high TG and low HDL-C by CardioChek PA analyzer were similar to those by ADVIA2400 automatic analyzer(P>0.05), but the rates of high LDL-C were lower than those by ADVIA2400 automatic analyzer(P<0.05). Sigma performance verification showed that test of LDL-C by CardioChek PA analyzer was unacceptable(σ<2). Conclusion Different blood collection methods had no effect on LDL-C results by CardioChek PA analyzer. The LDL-C values of CardioChek PA were lower than those of ADVIA2400 automatic analyzer, which indicated that prevention and treatment of ischemic stroke still needs to refer to the LDL-C values measured by automatic biochemical analyzer.

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备注/Memo

备注/Memo:
收稿日期: 2019-01-09 基金项目: 福建省科技重大专项(2017YZ0001-1); 福建省卫生厅中青年骨干人才培养项目(2015-ZQN-JC-21) 作者简介: 方 玲,女,主任医师,副教授,医学博士.Email:paulinefz@126.com
更新日期/Last Update: 2020-01-30