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[1]陈子若,杨 庭,余丽丽,等.原发性肝细胞癌患者血清抗p53抗体的检测及意义探讨[J].福建医科大学学报,2020,54(01):36-39.
 CHEN Ziruo,YANG Ting,YU Lili,et al.The Retection and Significance of Serum Anti-p53 Antibody for The Patients with Hepatocellular Carcinoma[J].Journal of Fujian Medical University,2020,54(01):36-39.
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第54卷
期数:
2020年01期
页码:
36-39
栏目:
临床研究
出版日期:
2020-02-28

文章信息/Info

Title:
The Retection and Significance of Serum Anti-p53 Antibody for The Patients with Hepatocellular Carcinoma
文章编号:
1672-4194(2020)01-0036-04
作者:
陈子若12 杨 庭14 余丽丽13 吴庆伟13 李亚东13 邱福南15 雷 棽13 黄 毅13
1.福建医科大学 省立临床医学院,福州 350001; 2.福建省立医院 核医学科,福州 350001; 3.福建省立医院 检验科,福州 350001; 4.福建省建新医院 检验科,福州 350002; 5.福建省立医院 肝胆外科,福州 350001
Author(s):
CHEN Ziruo12 YANG Ting14 YU Lili13 WU Qingwei13 LI Yadong13 QIU Funan15 LEI Shen13 HUANG Yi13
1.Provincial Clinical College, Fujian Medical University, Fuzhou 350001,China; 2.Department of Nuclear Medicine, Fujian Provincial Hospital, Fuzhou 350001,China; 3.Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001,China; 4.Department of Clinical Laboratory, Fujian Jianxin Hospital, Fuzhou 350002,China; 5.Department of Hepatobiliary Surgery, Fujian Provincial Hospital, Fuzhou 350001,China
关键词:
肝细胞 肿瘤 基因 p53 甲胎蛋白类
Keywords:
carcinoma hepatocellular neoplasms genes p53 alpha-fetoproteins
分类号:
R341.33; R730.261; R735.7
DOI:
-
文献标志码:
A
摘要:
目的 研究抗p53抗体检测以及与甲胎蛋白(AFP)联合检测对原发性肝细胞癌(HCC)诊断的效能。 方法 分别采用ELISA法与电化学发光法检测50例HCC、50例肝脏良性病变(LBL)、50例健康对照(HC)患者的血清抗p53抗体与AFP。 结果 HCC组血清抗p53抗体的水平和阳性率均明显高于LBL组(P<0.05)和HC组(P<0.01)。抗p53抗体对HCC的敏感性为36.0%,特异性为91.0%,ROC曲线下面积(AUC)为0.630(95% CI 0.547~0.707); AFP对HCC的敏感性为54.0%,特异性为83.0%,AUC为0.685(95%CI 0.604~0.758); 抗p53抗体与AFP联合检测可将对HCC敏感性提高至76.0%(特异性80.0%),AUC提高至0.770(95% CI 0.694~0.835)。 结论 抗p53抗体对HCC具有理想诊断价值; 抗p53抗体与AFP联合检测有助于提高对HCC的诊断效能(在保证特异性的前提下,提高敏感性)。
Abstract:
Objective To study the diagnostic performances of serum anti-p53 antibody(anti-p53)detection as well as combined detection of serum anti-p53 with(AFP)for hepatocellular carcinoma(HCC). Methods Serum anti-p53 and AFP of 50 patients with HCC, 50 patients with liver benign lesion(LBL)and 50 healthy controls(HC)were detected by enzyme linked immunosorbent assay(ELISA)and electrochemiluminescence immunoassay(ECL), respectively. Results The level and positive rate of serum anti-p53 in HCC group were significantly higher than that in LBL group(P<0.05)and HC group(P<0.01). The sensitivity, specificity and area under ROC curve(AUC)of serum anti-p53 for HCC were 36.0%, 91.0% and 0.630(95%CI 0.547-0.707), respectively. The sensitivity, specificity and AUC of serum AFP for HCC were 54.0%, 83.0% and 0.685(95%CI 0.604-0.758), respectively. The sensitivity and AUC of combined detection of anti-p53 with AFP for HCC was improved to 76.0%(specificity 80.0%)and 0.770(95%CI 0.694-0.835), respectively. Conclusions Anti-p53 has an ideal diagnostic value for HCC. The combined detection of anti-p53 with AFP might contribute to the marked improvement of diagnostic performance for HCC, with the evidence of improved sensitivity and enough high specificity for HCC.

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备注/Memo

备注/Memo:
收稿日期: 2018-11-26 基金项目: 福建省科技创新联合资金项目(2017Y9069); 福建省立医院高水平医院建设科研联合基金(2017LHJJ04) 作者简介: 陈子若,女,技师 通讯作者: 黄 毅. Email: hyi8070@126.com
更新日期/Last Update: 2020-01-30