|本期目录/Table of Contents|

[1]许晓文,洪瑞霞,杨丽娟,等.一测多评法测定复方金线莲中4种活性成分的含量[J].福建医科大学学报,2020,54(04):213-219224.
 XU Xiaowen,HONG Ruixia,YANG Lijuan,et al.Determination of Four Active Ingredients in Compound Anoectochilus roxburghii(Wall.)Lindl by QAMS[J].Journal of Fujian Medical University,2020,54(04):213-219224.
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第54卷
期数:
2020年04期
页码:
213-219224
栏目:
论著
出版日期:
2020-09-10

文章信息/Info

Title:
Determination of Four Active Ingredients in Compound Anoectochilus roxburghii(Wall.)Lindl by QAMS
文章编号:
1672-4194(2020)04-0213-08
作者:
许晓文 洪瑞霞 杨丽娟 黄丽英
福建医科大学 药学院,福州350122
Author(s):
XU Xiaowen HONG Ruixia YANG Lijuan HUANG Liying
School of Pharmacy, Fujian Medical University, Fuzhou 350122, China
关键词:
复方金线莲口服液 兰科 色谱法高压液相 鸟嘌呤/类似物和衍生物
Keywords:
Anoectochilus roxburghii oral liquid chromatography high pressure liquid guanine/analogs & derivatives
分类号:
R282.71; R916.4
DOI:
-
文献标志码:
A
摘要:
目的建立一测多评法同时测定复方金线莲口服液中尿苷、鸟苷、赤芝酸C和灵芝酸A的含量,并验证该方法的准确性。方法采用HPLC法,色谱条件:ZOBAX SB-Aq C 18柱(4.6 mm×250 mm,5 μm),流动相:乙腈-乙酸铵(含13 mmol/L乙酸铵、pH=4)为流动相,梯度洗脱; 流速1.0 mL/min,检测波长254 nm,柱温 30 ℃,进样量20 μL。以鸟苷为参照物,建立其对尿苷、赤芝酸C和灵芝酸A的相对校正因子,并用该校正因子进行尿苷、赤芝酸C、灵芝酸A的含量计算,其定量结果与外标法定量结果比较,以验证一测多评法的准确性和可行性。结果尿苷、鸟苷、赤芝酸C、灵芝酸A分别在2~40,3~60,1~20和 1~160 μg/mL范围内呈现良好线性关系(均为 r2=0.999 9); 尿苷、赤芝酸C、灵芝酸A的相对校正因子分别为1.02,0.34,0.40; 一测多评法的计算结果和外标法实测值之间无显著差异(S=0.999 9); 尿苷、鸟苷、赤芝酸C、灵芝酸A平均加样回收率(n=3)分别为94.5%,104.0%,86.6%和105.0%。结论建立的一测多评法可作为测定复方金线莲口服液中4种有效成分含量的测定方法。
Abstract:
ObjectiveTo establish a quantitative analysis of multi-components by single-marker(QAMS)for simultaneously determining uridine, guanosine, lucidenic acid C and ganoderic acid A in Compound Anoectochilus roxburghii(Wall.)Lindl (A.roxburghii)oral liquid, and validate its feasibility for quality evaluation.MethodsThe contents of uridine, guanosine, lucidenic acid C and ganoderic acid A in oral liquid were determined by high-performance liquid chromatography(HPLC), and the separation was performed on ZOBAX SB-Aq C 18 column(4.6 mm×250 mm, 5 μm).The mobile phase comprised acetonitrile-ammonium acetate(13 mmol/L ammonium acetate, pH=4)with the gradient elution and at a flow rate of 1.0 mL/min.The wavelength for detection and column temperature were set at 254 nm and 30 ℃, respectively.The injection volume was 20 μL.Using guanosine as the internal reference substance the relative correction factors(RCFs)for uridine, lucidenic acid C and ganoderic acid A was determined by HPLC-UV with good reproducibility.To verify the accuracy and feasibility of the QAMS, the contents of four active ingredients were also determined by the external standard method(ESM).ResultsThe linear range of uridine, guanosine, lucidenic acid C and ganoderic acid A were 2-40 μg/mL( r2=0.999 9), 3-60 μg/mL(r2=0.999 9), 1-20 μg/mL(r2=0.999 9)and 1-160 μg/mL( r2=0.999 9), respectively.RCFs of uridine, lucidenic acid C and ganoderic acid A with reference to guanosine were 1.02, 0.34, 0.40, respectively.No significant differences between the quantitative results of QAMS and ESM were observed(S=0.999 9).The average recoveries(n=3)were 94.5%, 104.0%, 86.6% and 105.0%, respectively.ConclusionThe established QAMS cab be used for the determination of four active ingredients in compound A.roxburghii oral liquid.

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备注/Memo

备注/Memo:
收稿日期: 2019-12-18
基金项目: 福建省科技厅对外合作重点项目(2016I0004); 福建省财政厅专项科研项目(2016B007)
作者简介: 许晓文,男,福建医科大学2018级硕士研究生
通讯作者: 黄丽英.Email:fjmuhly88@sina.com
更新日期/Last Update: 2020-10-15