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[1]林靓,柳之彦,林容,等.TNFAIP8在子痫前期胎盘组织中的甲基化情况及表达[J].福建医科大学学报,2020,54(04):255-259.
 LIN Liang,LIU Zhiyan,LIN Rong,et al.DNA Methylation Profile and Expression of Tumor Necrosis Factor Alpha Induced Protein 8(TNFAIP8)in Preeclampsia Placenta[J].Journal of Fujian Medical University,2020,54(04):255-259.
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TNFAIP8在子痫前期胎盘组织中的甲基化情况及表达(PDF)
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《福建医科大学学报》[ISSN:1672-4194/CN:35-1192/R]

卷:
第54卷
期数:
2020年04期
页码:
255-259
栏目:
临床研究
出版日期:
2020-09-10

文章信息/Info

Title:
DNA Methylation Profile and Expression of Tumor Necrosis Factor Alpha Induced Protein 8(TNFAIP8)in Preeclampsia Placenta
文章编号:
1672-4194(2020)04-0255-05
作者:
林靓 柳之彦 林容 阮舆鑫 董洁琼 郑晶
福建医科大学 省立临床医学院,福建省立医院 妇产科,福州350001
Author(s):
LIN Liang LIU Zhiyan LIN Rong RUAN Yuxin DONG Jieqiong ZHENG Jing
Department of Gynecology and Obstetrics, Provincial Clinic College of Fujian Medical University,Fujian Provincial Hospital, Fuzhou 350001, China
关键词:
先兆子痫 甲基化 肿瘤坏死因子类 基因
Keywords:
pre-eclampsia methylation tumor necrosis-factors genes
分类号:
R714.24; R714.244; R977.6
DOI:
-
文献标志码:
A
摘要:
目的探讨子痫前期胎盘组织中TNFAIP8甲基化情况及其在胎盘和外周血的表达水平。方法应用Illumina Human 450K甲基化芯片检测早发型组(EOPE组)、晚发型组(LOPE组)及对照组胎盘组织,焦磷酸测序验证TNFAIP8甲基化差别情况。采用RT-PCR检测TNFAIP8-mRNA,Western-blot检测TNFAIP8蛋白,进一步验证其在胎盘和外周血中的表达。结果与对照组比较,甲基化芯片检测和焦磷酸测序验证检测结果均提示LOPE组及EOPE组TNFAIP8甲基化水平均明显降低,差别有统计学意义(P<0.05); 而LOPE组与对照组比较,差别无统计学意义(P>0.05)。3组胎盘组织及外周血的TNFAIP8-mRNA及蛋白表达差别均有统计学意义(P<0.05)。结论TNFAIP8基因在EOPE胎盘组织中明显低甲基化。子痫前期胎盘及外周血中 TNFAIP8-mRNA及蛋白显著过表达,可能参与子痫前期发病机制。
Abstract:
ObjectiveTo explore the methylation profile and protein expression of TNFAIP8 in both the preeclampsia placenta and peripheral blood.MethodsThe placental tissues of early-onset preeclampsia(EOPE), late-onset preeclampsia(LOPE)and normal pregnant group(NP)were tested by Illumina Human 450K methylation beadchip.Differences in TNFAIP8 methylation profile among three groups were verified by pyrosequencing.The expression levels of TNFAIP8 mRNA and protein expression were examined by RT-PCR and Western-blot analysis.ResultsBoth the methylation beadchip analysis and pyrosequencing showed that TNFAIP8 hypomethylation in EOPE group was significantly different compared with LOPE group or NP group(P<0.05).However, there was no significant difference between LOPE group and NP group(P>0.05).The three groups have statistically significant difference in TNFAIP8 mRNA and protein expression(P<0.05).ConclusionsSignificant hypomethylation of TNFAIP8 occurs in placenta of early-onset preeclampsia.NFAIP8 mRNA and protein overexpression are probably associated with preeclampsia pathogenesis.

参考文献/References:

[1]Nandor G T,Roberto R,Adi L T,et al. Integrated systems biology approach identifies novel maternal and placental pathways of preeclampsia[J]. Front Immunol,2018,9:1661.
[2]Zhao S Y, Lv N, Li Y,et al. Identification and characterization of methylation-mediated transcriptional dysregulation dictate methylation roles in preeclampsia[J].Hum Genomics,2020,14(1): 5.
[3]林靓,余艳红,杨茵,等.子痫前期胎盘组织甲基化情况及免疫相关候选基因分析[J].中国妇幼保健杂志,2014,12(29):5624-5627.
[4]Boris N,Thierry F,Lynda K,et al. Increased methylation and decreased expression of homeobox genes TLX1, HOXA10 and DLX5 in human placenta are associated with trophoblast differentiation[J]. Sci Rep,2017,7(1):4523.
[5]Benjamin T M,Shalem Y L,Alicia K S,et al. Accelerated placental aging in early onset preeclampsia pregnancies identified by DNA methylation[J]. Epigenomics,2017,9(3):279-289.
[6]Pia M V,Pekka M,Jussi G,et al. Cluster analysis to estimate the risk of preeclampsia in the high-risk Prediction and Prevention of Preeclampsia and Intrauterine Growth Restriction(PREDO)study[J]. PLoS One,2017,12(3):e0174399.
[7]Patel S,Wang F H,Whiteside T L,et al.Identification of seven differentially displayed transcripts in human primary and matched metastatic head and neck squamous cell carcinoma cell lines:implications in metastasis and/or radiation response[J].Oral Oncol,1997,33:197-203.
[8]Zhang L,Liu R,Luan Y Y,et al. Tumor necrosis factor-α induced protein 8: Pathophysiology, clinical significance, and regulatory mechanism[J]. Int J Biol Sci, 2018,14(4): 398-405.
[9]Suryakant N,Dong X,Elena A,et al. Oncogenic role of tumor necrosis factor α-induced protein 8(TNFAIP8)[J]. Cells,2019,8(1):9.)
[10]Wu S,Li W,Wu Z,et al. TNFAIP8 promotes cisplatin resistance in cervical carcinoma cells by inhibiting cellular apoptosis[J]. Oncol Lett,2019,17(5):4667-4674.
[11]Jason R G,Youhai H C. Regulation of inflammation and tumorigenesis by the TIPE family of phospholipid transfer proteins[J]. Cell Mol Immunol,2017,14(6):482-487.
[12]Zhang L J,Liu X,Gafken P R. A chicken ovalbumin upstream promoter transcription factor I(COUP-TFI)complex represses expression of the gene encoding tumor necrosis factor α-induced protein 8(TNFAIP8)[J]. J Biol Chem,2008,284(10):6156-6168.
[13]Laskowska M.Altered maternal serum matrix metalloproteinases MMP-2, MMP-3, MMP-9, and MMP-13 in severe early- and late-onset preeclampsia[J].Biomed Res Int,2017,2017:6432426.
[14]Mousa A A,Cappello R E,Estrada-Gutierrez G,et al. Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism[J]. Am J Pathol,2012,181(4):1455-1463.
[15]Xiang Y,Zhang X,Li Q,et al. Promoter hypomethylation of TIMP3 is associated with pre-eclampsia in a Chinese population[J]. Mol Hum Reprod,2013,19(3):153-159.

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备注/Memo

备注/Memo:
收稿日期: 2019-07-16
基金项目: 福建省卫生计生科研人才培养项目(中青年骨干人才培养项目,2018-ZQN-22); 福建省立医院“创双高”火石基金项目(2020HSJJ19); 国家自然科学基金青年基金项目(81800363)作者简介: 林靓,女,主任医师,医学博士. Email: kitty342@163.com
更新日期/Last Update: 2020-10-15